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Validation Data Gallery

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  • Western Blot (WB) analysis of recombinant protein using AGBL5/CCP5 Polyclonal antibody (15569-1-AP)

    WB analysis of recombinant protein using 15569-1-AP

    WB result of 15569-1-AP (anti-CCP5): Fusion protein expressed in Baculovirus.(From Dr. Jason Ford).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using AGBL5/CCP5 Polyclonal antibody (15569-1-AP)

    IHC staining of mouse cerebellum using 15569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15569-1-AP (AGBL5/CCP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using AGBL5/CCP5 Polyclonal antibody (15569-1-AP)

    IHC staining of mouse cerebellum using 15569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15569-1-AP (AGBL5/CCP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AGBL5/CCP5 Polyclonal antibody (15569-1-AP)

    IHC staining of mouse brain using 15569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15569-1-AP (AGBL5/CCP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AGBL5/CCP5 Polyclonal antibody (15569-1-AP)

    IHC staining of mouse brain using 15569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15569-1-AP (AGBL5/CCP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AGBL5/CCP5 Polyclonal antibody

AGBL5/CCP5 Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 15569-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AGBL5, AGBL5/CCP5, ATP/GTP binding protein like 5, CCP5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrecombinant protein
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15569-1-AP targets AGBL5/CCP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7889

Product name: Recombinant human AGBL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 466-816 aa of BC007415

Sequence: SLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHSYTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFPSRYTVELFEQVGRAMAIAALDMAECNPWPRIVLSEHSSLTNLRAWMLKHVRNSRGLSSTLNVGVNKKRGLRTPPKSHNGLPVSCSENTLSRARSFSTGTSAGGSSSSQQNSPQMKNSPSFPFHGSRPAGLPGLGSSTQKVTHRVLGPVREPRSQDRRRQQQPLNHRPAGSLAPSPAPTSSGPASSHKLGSCLLPDSFNIPGSSCSLLSSGDKPEAVMVIGKGLLGTGARMPCIKTRLQTCPRRVSARRGPGFPRLGPGWAGAHRRLAEG

相同性解析による交差性が予測される生物種
Full Name ATP/GTP binding protein-like 5
Calculated molecular weight98 kDa
GenBank accession numberBC007415
Gene Symbol AGBL5/CCP5
Gene ID (NCBI) 60509
RRIDAB_2878151
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NDL9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for AGBL5/CCP5 antibody 15569-1-APDownload protocol
IHC protocol for AGBL5/CCP5 antibody 15569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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