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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using AFM Monoclonal antibody (66113-1-Ig)

    WB analysis of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66113-1-Ig (AFM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using AFM Monoclonal antibody (66113-1-Ig)

    IP experiment of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    IP result of anti-AFM (IP:66113-1-Ig, 5ug; Detection:66113-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AFM Monoclonal antibody (66113-1-Ig)

    IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

AFM Monoclonal antibody, PBS Only

AFM Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66113-1-PBS
Clone No.2G9F6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ALF, ALBA, ALB2, afamin/AFM, afamin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66113-1-PBS targets AFM in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag16866

Product name: Recombinant human AFM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 264-599 aa of BC109020

Sequence: SNYDGCCEGDVVQCIRDTSKVMNHICSKQDSISSKIKECCEKKIPERGQCIINSNKDDRPKDLSLREGKFTDSENVCQERDADPDTFFAKFTFEYSRRHPDLSIPELLRIVQIYKDLLRNCCNTENPPGCYRYAEDKFNETTEKSLKMVQQECKHFQNLGKDGLKYHYLIRLTKIAPQLSTEELVSLGEKMVTAFTTCCTLSEEFACVDNLADLVFGELCGVNENRTINPAVDHCCKTNFAFRRPCFESLKADKTYVPPPFSQDLFTFHADMCQSQNEELQRKTDRFLVNLVKLKHELTDEELQSLFTNFANVVDKCCKAESPEVCFNEESPKIGN

相同性解析による交差性が予測される生物種
Full Name afamin
Calculated molecular weight 599 aa, 69 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC109020
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2881512
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP43652
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

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