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Validation Data Gallery

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  • Western Blot (WB) analysis of human blood using AFM Polyclonal antibody (25280-1-AP)

    WB analysis of human blood using 25280-1-AP (same clone as 25280-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 25280-1-AP (AFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25280-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using AFM Polyclonal antibody (25280-1-AP)

    IP experiment of human plasma using 25280-1-AP (same clone as 25280-1-PBS)

    IP result of anti-AFM (IP:25280-1-AP, 4ug; Detection:25280-1-AP 1:2000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 25280-1-PBS in a different storage buffer formulation.

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AFM Polyclonal antibody, PBS Only

AFM Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 25280-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25280-1-PBS targets AFM in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFM fusion protein Ag16866 相同性解析による交差性が予測される生物種
Full Name afamin
Calculated molecular weight 599 aa, 69 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC109020
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2880005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43652
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.