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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AFF2 Polyclonal antibody (25414-1-AP)

    WB analysis using 25414-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25414-1-AP (AFF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using AFF2 Polyclonal antibody (25414-1-AP)

    IP experiment of SH-SY5Y using 25414-1-AP

    IP result of anti-AFF2 (IP:25414-1-AP, 4ug; Detection:25414-1-AP 1:600) with SH-SY5Y cells lysate 1720 ug.

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AFF2 Polyclonal antibody

AFF2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25414-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

AF4/FMR2 family member 2, FMR2, FMR2P, FRAXE, MRX2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IP detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25414-1-AP targets AFF2 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFF2 fusion protein Ag22225 相同性解析による交差性が予測される生物種
Full Name AF4/FMR2 family, member 2
Calculated molecular weight 1311 aa, 145 kDa
Observed molecular weight145 kDa
GenBank accession numberBC132683
Gene Symbol AFF2
Gene ID (NCBI) 2334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51816
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AFF2 (OMIM* 300806) (also known as FMR2 gene), which encodes AF4/FMR2 family member 2, is a transcriptional factor and RNA-binding protein that plays an important role in transcriptional regulation, RNA splicing, mRNA processing, and nuclear speckle organization . AFF2 is highly conserved and abundantly expressed in human brain, being essential for brain development. Homozygous AFF2 knock-out mice showed abnormal central nervous system synaptic transmission, abnormal excitatory postsynaptic potential, and premature death (PMID: 35431806).

Protocols

Product Specific Protocols
WB protocol for AFF2 antibody 25414-1-APDownload protocol
IP protocol for AFF2 antibody 25414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols