Validation Data Gallery
Tested Applications
Positive WB detected in | SH-SY5Y cells |
Positive IP detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
25414-1-AP targets AFF2 in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AFF2 fusion protein Ag22225 相同性解析による交差性が予測される生物種 |
Full Name | AF4/FMR2 family, member 2 |
Calculated molecular weight | 1311 aa, 145 kDa |
Observed molecular weight | 145 kDa |
GenBank accession number | BC132683 |
Gene Symbol | AFF2 |
Gene ID (NCBI) | 2334 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P51816 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AFF2 (OMIM* 300806) (also known as FMR2 gene), which encodes AF4/FMR2 family member 2, is a transcriptional factor and RNA-binding protein that plays an important role in transcriptional regulation, RNA splicing, mRNA processing, and nuclear speckle organization . AFF2 is highly conserved and abundantly expressed in human brain, being essential for brain development. Homozygous AFF2 knock-out mice showed abnormal central nervous system synaptic transmission, abnormal excitatory postsynaptic potential, and premature death (PMID: 35431806).
Protocols
Product Specific Protocols | |
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WB protocol for AFF2 antibody 25414-1-AP | Download protocol |
IP protocol for AFF2 antibody 25414-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |