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Validation Data Gallery

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  • Western Blot (WB) analysis of COS-7 cells using AFAP1L1 Polyclonal antibody (23909-1-AP)

    WB analysis of COS-7 using 23909-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 23909-1-AP (AFAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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AFAP1L1 Polyclonal antibody

AFAP1L1 Polyclonal Antibody for WB, ELISA
Cat No. 23909-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, ELISA

Synonyms

AFAP1 like protein 1, AFAP1L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOS-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23909-1-AP targets AFAP1L1 in WB, ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFAP1L1 fusion protein Ag21006 相同性解析による交差性が予測される生物種
Full Name actin filament associated protein 1-like 1
Calculated molecular weight 768 aa, 86 kDa
Observed molecular weight 75-86 kDa
GenBank accession numberBC125093
Gene Symbol AFAP1L1
Gene ID (NCBI) 134265
RRIDAB_2879358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8TED9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Actin Filament-Associated Protein of 110 kDa (AFAP-110/AFAP1) is an adaptor protein with multiple protein binding motifs that is known to function as both an actin binding protein and a cSrc activating protein. A homology search of AFAP1 recently identified AFAP1L1 which has a similar sequence, domain structure and cellular localization. AFAP1L1 is highly expressed in human breast, colon and brain tissue. AFAP1L1 interacts with cortactin and involves the invadosomes formation. AFAP1L1 as a prognostic marker has a role in the progression of spindle cell sarcomas.

Protocols

Product Specific Protocols
WB protocol for AFAP1L1 antibody 23909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols