Validation Data Gallery
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Tested Applications
| Positive WB detected in | HL-60 cells, Ramos cells, NIH/3T3 cells, C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86131-1-RR targets ADSL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ADSL fusion protein Ag7332 相同性解析による交差性が予測される生物種 |
| Full Name | adenylosuccinate lyase |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC000253 |
| Gene Symbol | ADSL |
| Gene ID (NCBI) | 158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P30566 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Adenylosuccinate lyase (ADSL) is an essential enzyme for de novo purine biosynthesis and the purine nucleotide cycle, where it plays a key role in the regulation of adenosine monophosphate levels in the cells. Given its crucial role in both cellular replication and metabolism, it is not surprising that ADSL has been found dysregulated in several malignancies, including breast and endometrial cancers as well as glioma. (PMID: 33754045)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADSL antibody 86131-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |