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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADRP Recombinant antibody

ADRP Recombinant Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230274F4

Cat no : 80362-2-RR

Synonyms

ADFP, Adipophilin, ADRP, Perilipin 2, PLIN2

Validation Data Gallery

Applications:
  • WB analysis using 80362-2-RR

    WB analysis using 80362-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 80362-2-RR

    WB analysis using 80362-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 80362-2-RR

    IF Staining of HeLa using 80362-2-RR

    Immunofluorescent analysis of (4% PFA) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 80362-2-RR

    FC experiment of HepG2 using 80362-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ADRP Recombinant antibody (80362-2-RR, Clone:230274F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inTHP-1 cells, HepG2 cells, K-562 cells
Positive IF detected inoleic acid treated HeLa cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ADRP Ready-To-Use IHC Kit. ADRP primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

80362-2-RR targets ADRP in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ADRP fusion protein Ag7539 相同性解析による交差性が予測される生物種
Full Name adipose differentiation-related protein
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC005127
Gene symbol ADFP
Gene ID (NCBI) 123
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Protocols

Product Specific Protocols
WB protocol for ADRP antibody 80362-2-RRDownload protocol
IF protocol for ADRP antibody 80362-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols