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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADRBK1 Monoclonal antibody (67067-1-Ig)

    WB analysis using 67067-1-Ig (same clone as 67067-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67067-1-Ig (ADRBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using ADRBK1 Monoclonal antibody (67067-1-Ig)

    WB analysis of U-937 using 67067-1-Ig (same clone as 67067-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 67067-1-Ig (ADRBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ADRBK1 Monoclonal antibody (67067-1-Ig)

    IHC staining of human lymphoma using 67067-1-Ig (same clone as 67067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ADRBK1 Monoclonal antibody (67067-1-Ig)

    IHC staining of human lymphoma using 67067-1-Ig (same clone as 67067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ADRBK1 Monoclonal antibody (67067-1-Ig)

    IHC staining of human tonsillitis using 67067-1-Ig (same clone as 67067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ADRBK1 Monoclonal antibody (67067-1-Ig)

    IHC staining of human tonsillitis using 67067-1-Ig (same clone as 67067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ADRBK1 Monoclonal antibody (67067-1-Ig)

    IHC staining of human tonsillitis using 67067-1-Ig (same clone as 67067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ADRBK1 Monoclonal antibody (67067-1-Ig)

    IHC staining of human tonsillitis using 67067-1-Ig (same clone as 67067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ADRBK1 Monoclonal antibody (67067-1-Ig)

    IF Staining of Jurkat using 67067-1-Ig (same clone as 67067-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ADRBK1 antibody (67067-1-Ig, Clone: 2F7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ADRBK1 Monoclonal antibody (67067-1-Ig)

    FC experiment of HeLa using 67067-1-Ig (same clone as 67067-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ADRBK1 (67067-1-Ig, Clone:2F7A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

ADRBK1 Monoclonal antibody, PBS Only

ADRBK1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67067-1-PBS
Clone No.2F7A5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

ARBK1, GRK2, BARK, BARK1, Beta ARK 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67067-1-PBS targets ADRBK1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5071

Product name: Recombinant human ADRBK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 390-689 aa of BC037963

Sequence: PFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

相同性解析による交差性が予測される生物種
Full Name adrenergic, beta, receptor kinase 1
Calculated molecular weight 689 aa, 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC037963
Gene Symbol ADRBK1
Gene ID (NCBI) 156
RRIDAB_2882377
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP25098
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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