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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ADORA2A Polyclonal antibody (51092-1-AP)

    WB analysis of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ADORA2A Polyclonal antibody (51092-1-AP)

    WB analysis using 51092-1-AP (same clone as 51092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using ADORA2A Polyclonal antibody (51092-1-AP)

    WB analysis of rat brain using 51092-1-AP (same clone as 51092-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ADORA2A Polyclonal antibody (51092-1-AP)

    WB analysis using 51092-1-AP (same clone as 51092-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ADORA2A Polyclonal antibody (51092-1-AP)

    IHC staining of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ADORA2A Polyclonal antibody (51092-1-AP)

    IHC staining of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

ADORA2A Polyclonal antibody, PBS Only

ADORA2A Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 51092-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, Indirect ELISA

Synonyms

adenosine A2a receptor, Adenosine receptor A2a, ADORA2, RDC8

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51092-1-PBS targets ADORA2A in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0470

Product name: Recombinant human ADORA2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 300-412 aa of BC013780

Sequence: RKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

相同性解析による交差性が予測される生物種
Full Name adenosine A2a receptor
Calculated molecular weight 412 aa, 45 kDa
Observed molecular weight 38 kDa, 40-45 kDa
GenBank accession numberBC013780
Gene Symbol ADORA2A
Gene ID (NCBI) 135
RRIDAB_2881245
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP29274
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Adenosine (ADO) is a retaliatory metabolite that is expressed in conditions of injury or stress. Its physiological functions are mediated through interaction with four specific transmembrane receptors called ADORA1, ADORA2A, ADORA2B, and ADORA3 (PMID:23994158). ADORA2A (adenosine A2A receptor) has been shown to dampen inflammatory responses. ADORA2A signaling has been discovered on polymorphonuclear neutrophils (PMNs) (PMID:36009485).

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