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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ADO Polyclonal antibody (16479-1-AP)

    WB analysis of human brain using 16479-1-AP (same clone as 16479-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ADO Polyclonal antibody (16479-1-AP)

    WB analysis using 16479-1-AP (same clone as 16479-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using ADO Polyclonal antibody (16479-1-AP)

    WB analysis of mouse spleen using 16479-1-AP (same clone as 16479-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ADO Polyclonal antibody (16479-1-AP)

    IHC staining of human brain using 16479-1-AP (same clone as 16479-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ADO Polyclonal antibody (16479-1-AP)

    IHC staining of human brain using 16479-1-AP (same clone as 16479-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ADO Polyclonal antibody, PBS Only

ADO Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16479-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2 aminoethanethiol dioxygenase, 2-aminoethanethiol dioxygenase, C10orf22, Cysteamine dioxygenase, EC:1.13.11.19

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16479-1-PBS targets ADO in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9598

Product name: Recombinant human ADO protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-270 aa of BC018660

Sequence: MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGGGQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAPPYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP

相同性解析による交差性が予測される生物種
Full Name 2-aminoethanethiol (cysteamine) dioxygenase
Calculated molecular weight 270 aa, 30 kDa
Observed molecular weight 28 kDa-30 kDa
GenBank accession numberBC018660
Gene Symbol ADO
Gene ID (NCBI) 84890
RRIDAB_2222204
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SZ5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ADO, or 2-aminoethanethiol dioxygenase, cysteamine dioxygenase, is a thiol dioxygenase in humans that adds two oxygen atoms to cysteamine, forming hypotaurine (PMID: 39637922). ADO has been reported to exhibit two distinct biological functions with a nonheme iron center. It catalyzes oxidation of both cysteamine in sulfur metabolism and N-terminal cysteine-containing proteins or peptides, such as regulator of G protein signaling 5 (RGS5) (PMID: 34508780). ADO is ubiquitously expressed with the highest levels in brain, heart, and skeletal muscle. Western blot analysis detected two bands at 28~30 kDa as indicated in publications (PMID: 17581819).

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