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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADK Monoclonal antibody

ADK Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1A5

Cat no : 66929-1-Ig

Synonyms

Adenosine 5 phosphotransferase, adenosine kinase, ADK, AK

Validation Data Gallery

Applications:
  • WB analysis using 66929-1-Ig

    WB analysis using 66929-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66929-1-Ig (ADK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66929-1-Ig

    IHC staining of human liver cancer using 66929-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66929-1-Ig

    IHC staining of human liver cancer using 66929-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 66929-1-Ig

    IF Staining of human liver cancer using 66929-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human breast cancer using 66929-1-Ig

    IF Staining of human breast cancer using 66929-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of NIH/3T3 using 66929-1-Ig

    FC experiment of NIH/3T3 using 66929-1-Ig

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human ADK (66929-1-Ig, Clone:1G1A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue, human breast cancer tissue
Positive FC detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66929-1-Ig targets ADK in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADK fusion protein Ag7466 相同性解析による交差性が予測される生物種
Full Name adenosine kinase
Calculated molecular weight 41 kDa
Observed molecular weight 40-46 kDa
GenBank accession numberBC003568
Gene symbol ADK
Gene ID (NCBI) 132
RRIDAB_2882255
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ADK antibody 66929-1-IgDownload protocol
IHC protocol for ADK antibody 66929-1-IgDownload protocol
IF protocol for ADK antibody 66929-1-IgDownload protocol
FC protocol for ADK antibody 66929-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Authors - Nicole M Sayles
View Article
human,mouseWB,IHC

Gastroenterology

Hepatocyte Adenosine Kinase Promotes Excessive Fat Deposition and Liver Inflammation

Authors - Honggui Li
View Article
mouseWB

EMBO J

Regulation of LRRK2 mRNA stability by ATIC and its substrate AICAR through ARE-mediated mRNA decay in Parkinson's disease

Authors - Qinfang Liu
View Article