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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADK Polyclonal antibody (15477-1-AP)

    WB analysis using 15477-1-AP (same clone as 15477-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15477-1-AP (ADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADK Polyclonal antibody (15477-1-AP)

    IHC staining of human liver cancer using 15477-1-AP (same clone as 15477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15477-1-AP (ADK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADK Polyclonal antibody (15477-1-AP)

    IHC staining of human liver cancer using 15477-1-AP (same clone as 15477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15477-1-AP (ADK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15477-1-PBS in a different storage buffer formulation.

ADK Polyclonal antibody, PBS Only

ADK Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15477-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15477-1-PBS targets ADK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7783

Product name: Recombinant human ADK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-345 aa of BC003568

Sequence: MTSVRENILFGMGNPLLDISAVVDKDFLDKYSLKPNDQILAEDKHKELFDELVKKFKVEYHAGGSTQNSIKVAQWMIQQPHKAATFFGCIGIDKFGEILKRKAAEAHVDAHYYEQNEQPTGTCAACITGDNRSLIANLAAANCYKKEKHLDLEKNWMLVEKARVCYIAGFFLTVSPESVLKVAHHASENNRIFTLNLSAPFFSQFYKESLMKVMPYVDILFGNETEAATFAREQGFETKDIKEIAKKTQALPKMNSKRQRIVIFTQGRDDTIMATESEVTAFAVLDQDQKEIIDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASIIIRRTGCTFPEKPDFH

相同性解析による交差性が予測される生物種
Full Name adenosine kinase
Calculated molecular weight 41 kDa
Observed molecular weight 40-46 kDa
GenBank accession numberBC003568
Gene Symbol ADK
Gene ID (NCBI) 132
RRIDAB_2222085
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP55263
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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