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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADH1C Polyclonal antibody (18897-1-AP)

    WB analysis using 18897-1-AP (same clone as 18897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18897-1-AP (ADH1C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IP experiment of mouse liver using 18897-1-AP (same clone as 18897-1-PBS)

    IP result of anti-ADH1C (IP:18897-1-AP, 4ug; Detection:18897-1-AP 1:1000) with mouse liver tissue lysate 4000 ug. This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human kidney using 18897-1-AP (same clone as 18897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of rat liver using 18897-1-AP (same clone as 18897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human lung cancer using 18897-1-AP (same clone as 18897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human lung cancer using 18897-1-AP (same clone as 18897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human colon using 18897-1-AP (same clone as 18897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human colon using 18897-1-AP (same clone as 18897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADH1C Polyclonal antibody (18897-1-AP)

    IF Staining of HepG2 using 18897-1-AP (same clone as 18897-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using ADH1C antibody 18897-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADH1C Polyclonal antibody (18897-1-AP)

    IF Staining of HepG2 using 18897-1-AP (same clone as 18897-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1C antibody (18897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

ADH1C Polyclonal antibody, PBS Only

ADH1C Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18897-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:1.1.1.1, ADH3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18897-1-PBS targets ADH1C in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13528

Product name: Recombinant human ADH1C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-374 aa of BC062476

Sequence: MSTAGKVIKCKAAVLWELKKPFSIEEVEVAPPKAHEVRIKMVAAGICRSDEHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRICKNPESNYCLKNDLGNPRGTLQDGTRRFTCSGKPIHHFVGVSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVKVAKVTPGSTCAVFGLGGVGLSVVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGQLDTMMASLLCCHEACGTSVIVGVPPDSQNLSINPMLLLTGRTWKGAIFGGFKSKESVPKLVADFMAKKFSLDALITNVLPFEKINEGFDLLRSGKSIRTVLT

相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 1C (class I), gamma polypeptide
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 37-45 kDa
GenBank accession numberBC062476
Gene Symbol ADH1C
Gene ID (NCBI) 126
RRIDAB_10596950
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP00326
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).

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