Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, mouse testis tissue, rat brain tissue, rat testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18163-1-AP targets ADCYAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12600 Product name: Recombinant human ADCYAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC093837 Sequence: MTMCSGARLALLVYGIIMHSSVYSSPAAAGLRFPGIRPEEEAYGEDGNPLPDFDGSEPPGAGSPASAPRAAAAWYRPAGRRDVAHGILNEAYRKVLDQLSAGKHLQSLVARGVGGSLGGGAGDDAEPLSKRHSDGIFTDSYSRYRKQMAVKKYLAAVLGKRYKQRVKNKGRRIAYL 相同性解析による交差性が予測される生物種 |
| Full Name | adenylate cyclase activating polypeptide 1 (pituitary) |
| Calculated molecular weight | 176 aa, 19 kDa |
| Observed molecular weight | 19 kDa |
| GenBank accession number | BC093837 |
| Gene Symbol | ADCYAP1 |
| Gene ID (NCBI) | 116 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18509 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ADCYAP1 gene encodes adenylate cyclase-activating polypeptide (PACAP), a member of the secretin/glucagon/growth releasing hormone family. PACAP is widely distributed in both the central nervous system and peripheral organs (PMID: 33449573). It exists in two isoforms derived from the same precursor: PACAP-38, consisting of 38 amino acids, and PACAP-27, consisting of 27 amino acids (PMID: 19805477). PACAP is known to be involved in various biological processes. Its diverse effects are mediated through binding to three G-protein-coupled receptors known as PAC1, VPAC1, and VPAC2 receptors. By binding to these receptors, PACAP can initiate various signaling pathways, including downstream adenylate cyclase (AC) or phospholipase-C (PLC) activation, as well as calcium-regulated mechanisms [4]. Thus, they are involved in neuronal death, inflammatory response, cellular fission and modulation of immune system (PMID: 40266460, PMID: 26702849).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ADCYAP1 antibody 18163-1-AP | Download protocol |
| IHC protocol for ADCYAP1 antibody 18163-1-AP | Download protocol |
| IP protocol for ADCYAP1 antibody 18163-1-AP | Download protocol |
| WB protocol for ADCYAP1 antibody 18163-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |