Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, mouse brain tissue, SKOV-3 cells |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| CoIP | See 1 publications below |
Product Information
14616-1-AP targets ADCY6 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6218 Product name: Recombinant human ADCY6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 310-655 aa of BC064923 Sequence: AEVSQRQAFQETRGYIQARLHLQHENRQQERLLLSVLPQHVAMEMKEDINTKKEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGVDMIEAISLVREVTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGRAGRIHITRATLQYLNGDYEVEPGRGGERNAYLKEQHIETFLILGASQKRKEEKAMLAKLQRTRANSMEGLMPRWVPDRAFSRTKDSKAFRQMGIDDSSKDNRGTQDALNPEDEVDEFLSRAIDARSIDQLRKDHVRRFLLTFQRE 相同性解析による交差性が予測される生物種 |
| Full Name | adenylate cyclase 6 |
| Calculated molecular weight | 131 kDa |
| Observed molecular weight | 130-150 kDa |
| GenBank accession number | BC064923 |
| Gene Symbol | ADCY6 |
| Gene ID (NCBI) | 112 |
| RRID | AB_2242343 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43306 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Adenylate cyclase 6 (ADCY6) belongs to the membrane-bound adenylate cyclase family. Increasing evidence has proven that ADCY6 is involved in pathophysiological processes of the body, such as the stability of cardiovascular system function, glucose and lipid metabolism, and even carcinogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ADCY6 antibody 14616-1-AP | Download protocol |
| WB protocol for ADCY6 antibody 14616-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca2 | ||
Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) | ||
J Cell Biol Sonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length | ||
Mol Pharmacol Activity of adenylyl cyclase type 6 is suppressed by direct binding of the cytoskeletal protein 4.1G. | ||
Pathol Oncol Res Knockdown of GPSM1 Inhibits the Proliferation and Promotes the Apoptosis of B-Cell Acute Lymphoblastic Leukemia Cells by Suppressing the ADCY6-RAPGEF3-JNK Signaling Pathway. |