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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADARB1 Monoclonal antibody (67764-1-Ig)

    WB analysis using 67764-1-Ig (same clone as 67764-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67764-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ADARB1 Monoclonal antibody (67764-1-Ig)

    WB analysis of HSC-T6 using 67764-1-Ig (same clone as 67764-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67764-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ADARB1 Monoclonal antibody (67764-1-Ig)

    WB analysis using 67764-1-Ig (same clone as 67764-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67764-1-PBS in a different storage buffer formulation.

ADARB1 Monoclonal antibody, PBS Only

ADARB1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67764-1-PBS
Clone No.1H8G3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Synonyms

ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67764-1-PBS targets ADARB1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17852

Product name: Recombinant human ADARB1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 176-392 aa of BC065545

Sequence: LFNGFETPDKAEPPFYVGSNGDDSFSSSGDLSLSASPVPASLAQPPLPALPPFPPPSGKNPVMILNELRPGLKYDFLSESGESHAKSFVMSVVVDGQFFEGSGRNKKLAKARAAQSALAAIFNLHLDQTPSRQPIPSEGLQLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMTTGTDVKDAKVISVSTGTKCINGEYMSDRGLALND

相同性解析による交差性が予測される生物種
Full Name adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
Calculated molecular weight 741 aa, 81 kDa
Observed molecular weight70 kDa, 80 kDa
GenBank accession numberBC065545
Gene Symbol ADARB1
Gene ID (NCBI) 104
RRIDAB_2918531
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP78563
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ADARB1 (Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).

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