Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, 4T1 cells, C2C12 cells, HeLa cells, NIH/3T3 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 12 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| RIP | See 2 publications below |
Product Information
22248-1-AP targets ADARB1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17743 Product name: Recombinant human ADARB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 176-392 aa of BC065545 Sequence: LFNGFETPDKAEPPFYVGSNGDDSFSSSGDLSLSASPVPASLAQPPLPALPPFPPPSGKNPVMILNELRPGLKYDFLSESGESHAKSFVMSVVVDGQFFEGSGRNKKLAKARAAQSALAAIFNLHLDQTPSRQPIPSEGLQLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMTTGTDVKDAKVISVSTGTKCINGEYMSDRGLALND 相同性解析による交差性が予測される生物種 |
| Full Name | adenosine deaminase, RNA-specific, B1 (RED1 homolog rat) |
| Calculated molecular weight | 741 aa, 81 kDa |
| Observed molecular weight | 74-86 kDa |
| GenBank accession number | BC065545 |
| Gene Symbol | ADARB1 |
| Gene ID (NCBI) | 104 |
| RRID | AB_2879047 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78563 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADARB1(Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADARB1 antibody 22248-1-AP | Download protocol |
| IP protocol for ADARB1 antibody 22248-1-AP | Download protocol |
| WB protocol for ADARB1 antibody 22248-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Basic Res Cardiol Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs. | ||
Sci Rep Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice. | ||
FASEB J Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling.
| ||
J Cell Mol Med Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis. | ||
Front Pharmacol Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma. |
