Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, K-562 cells, HUVEC cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31075-1-AP targets ADAMTS14 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34638 Product name: Recombinant human ADAMTS14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1086-1223 aa of NM_080722 Sequence: HRLCCVSCIKKASGPNPGPDPGPTSLPPFSTPGSPLPGPQDPADAAEPPGKPTGSEDHQHGRATQLPGALDTSSPGTQHPFAPETPIPGASWSISPTTPGGLPWGWTQTPTPVPEDKGQPGEDLRHPGTSLPAASPVT 相同性解析による交差性が予測される生物種 |
| Full Name | ADAM metallopeptidase with thrombospondin type 1 motif, 14 |
| Calculated molecular weight | 134 kDa |
| Observed molecular weight | 134 kDa |
| GenBank accession number | NM_080722 |
| Gene Symbol | ADAMTS14 |
| Gene ID (NCBI) | 140766 |
| RRID | AB_3669843 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8WXS8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation, and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS14 possesses high similarity with ADAMTS2 and ADAMTS3 in sequence and domain composition (PMID: 11741898). The expression of ADAMTS14 is correlated with the occurrence and progression of colorectal cancer and oral squamous cell carcinoma (PMID: 34856162; PMID: 32102222).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADAMTS14 antibody 31075-1-AP | Download protocol |
| WB protocol for ADAMTS14 antibody 31075-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |