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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse placenta tissue using ADAMTS12 Polyclonal antibody (24934-1-AP)

    WB analysis of mouse placenta using 24934-1-AP (same clone as 24934-1-PBS)

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 24934-1-AP (ADAMTS12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24934-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using ADAMTS12 Polyclonal antibody (24934-1-AP)

    WB analysis of NIH/3T3 using 24934-1-AP (same clone as 24934-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24934-1-AP (ADAMTS12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24934-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ADAMTS12 Polyclonal antibody (24934-1-AP)

    IF Staining of NIH/3T3 using 24934-1-AP (same clone as 24934-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ADAMTS12 antibody (24934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24934-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ADAMTS12 Polyclonal antibody, PBS Only

ADAMTS12 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24934-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24934-1-PBS targets ADAMTS12 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20712

Product name: Recombinant human ADAMTS12 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-229 aa of BC058841

Sequence: MPCAQRSWLANLSVVAQLLNFGALCYGRQPQPGPVRFPDRRQEHFIKGLPEYHVVGPVRVDASGHFLSYGLHYPITSSRRKRDLDGSEDWVYYRISHEEKDLFFNLTVNQGFLSNSYIMEKRYGNLSHVKMMASSAPLCHLSGTVLQQGTRVGTAALSACHGLTGFFQLPHGDFFIEPVKKHPLVEGGYHPHIVYRRQKVPETKEPTCGLKGIVTHMSSWVEESVLFFW

相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 12
Calculated molecular weight 178 kDa
Observed molecular weight 155 kDa
GenBank accession numberBC058841
Gene Symbol ADAMTS12
Gene ID (NCBI) 81792
RRIDAB_2879806
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP58397
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.

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