Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, rat cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32969-1-AP targets ADAM23 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35744 Product name: Recombinant human ADAM23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 342-488 aa of BC132765 Sequence: NTRVVLVAVETWTEKDQIDITTNPVQMLHEFSKYRQRIKQHADAVHLISRVTFHYKRSSLSYFGGVCSRTRGVGVNEYGLPMAVAQVLSQSLAQNLGIQWEPSSRKPKCDCTESWGGCIMEETGVSHSRKFSKCSILEYRDFLQRGG 相同性解析による交差性が予測される生物種 |
| Full Name | ADAM metallopeptidase domain 23 |
| Calculated molecular weight | 832 aa, 92 kDa |
| Observed molecular weight | 92 kDa, 70 kDa |
| GenBank accession number | BC132765 |
| Gene Symbol | ADAM23 |
| Gene ID (NCBI) | 8745 |
| RRID | AB_3742807 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O75077 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADAM23 (A disintegrin and metalloprotease protein 23), also known as MDC-3 (Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3) is a transmembrane type I glycoprotein involved with the development and maintenance of the nervous system, including neurite outgrowth, neuronal adhesion and differentiation and regulation of synaptic transmission. In addition, ADAM23 seems to participate in immune response and tumor establishment through interaction with different members of integrin receptors (PMID: 19692335). Two forms of ADAM23 have been previously described in primary cerebellar granule cells as an unprocessed precursor (100 kDa) and mature (70 kDa) protein, respectively (PMID: 29792904).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAM23 antibody 32969-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |