Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20755-1-AP targets ADAM20 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ADAM20 fusion protein Ag14493 相同性解析による交差性が予測される生物種 |
| Full Name | ADAM metallopeptidase domain 20 |
| Calculated molecular weight | 776 aa, 87 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC025378 |
| Gene Symbol | ADAM20 |
| Gene ID (NCBI) | 8748 |
| RRID | AB_3669344 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43506 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADAM20 is the functional equivalent of sperm fertilin-alpha (ADAM1), since that gene is nonfunctional in human. And northern blot analysis detected the 2.8-kb ADAM20 mRNA only in testis. The exclusive expression of ADAM20 in human testis and their sequence similarity with the fertilins suggest that it is expressed on sperm cells and involved in sperm maturation and/or fertilization(PMID:9469942). The full length protein has a singnal peptide, propeptide and 6 glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAM20 antibody 20755-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |