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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ADAM2 Polyclonal antibody (13952-1-AP)

    WB analysis of mouse testis using 13952-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ADAM2 Polyclonal antibody (13952-1-AP)

    WB analysis of mouse testis using 13952-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ADAM2 Polyclonal antibody (13952-1-AP)

    WB analysis of mouse testis using 13952-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using ADAM2 Polyclonal antibody (13952-1-AP)

    WB analysis of human testis using 13952-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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ADAM2 Polyclonal antibody

ADAM2 Polyclonal Antibody for WB, ELISA
Cat No. 13952-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ADAM 2, ADAM metallopeptidase domain 2, ADAM2, Cancer/testis antigen 15, CRYN1, CRYN2, CT15, Fertilin subunit beta, FTNB, PH 30, PH 30b, PH30, PH30 beta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13952-1-AP targets ADAM2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM2 fusion protein Ag5063 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 2
Calculated molecular weight 82 kDa
Observed molecular weight 50-55 kDa, 65-75 kDa
GenBank accession numberBC034957
Gene Symbol ADAM2
Gene ID (NCBI) 2515
RRIDAB_2877996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99965
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction(PMID: 27341348). Fertilin, a heterodimeric protein complex composed of a(ADAM1) and b(ADAM2) subunits present on the sperm surface, has been believed to mediate adhesion and fusion between the sperm and egg plasma membranes. ADAM2-deficient mouse sperm is also defective in adhesion to and fusion with the egg membrane (PMID:16407235). It has 2 isoforms produced by alternative splicing and 5 glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for ADAM2 antibody 13952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols