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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ADAM17 Rabbit Recombinant Antibody (98674-2-RR)

    FC experiment of human PBMCs using 98674-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ADAM17 Rabbit RecAb (98674-2-RR, Clone:251631A5), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ADAM17 Rabbit Recombinant Antibody (98674-2-RR)

    FC experiment of human PBMCs using 98674-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ADAM17 Rabbit RecAb (98674-2-RR, Clone:251631A5) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity of 98674-2-RR

    Affinity of 98674-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98674-2-RR against Human ADAM17 were performed. The affinity constant is 1.60 nM.

Anti-Human ADAM17 Rabbit Recombinant Antibody

ADAM17 Uni-rAb® Recombinant Antibody for FC
Cat No. 98674-2-RR
Clone No.251631A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86, Snake venom-like protease

Formulation:  PBS, Azide
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98674-2-RR targets ADAM17 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg4732

Product name: Recombinant Human ADAM17 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 470-570 aa of NM_003183.5

Sequence: FQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKEGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGNAEDDTVCLDL

相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 17
Calculated molecular weight93 kDa
GenBank accession numberNM_003183.5
Gene Symbol ADAM17
Gene ID (NCBI) 6868
RRIDAB_3746262
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP78536-1
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 mediates the release of pro-inflammatory cytokines and modulates cell signaling pathways involved in tumor progression and metastasis (PMID:17438092).

Protocols

Product Specific Protocols
FC protocol for ADAM17 antibody 98674-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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