Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 38 publications below |
| IHC | See 4 publications below |
| IF | See 8 publications below |
Product Information
25900-1-AP targets ADAM10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23078 Product name: Recombinant human ADAM10 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 306-512 aa of BC126253 Sequence: EQNHDDYCLAYVFTDRDFDDGVLGLAWVGAPSGSSGGICEKSKLYSDGKKKSLNTGIITVQNYGSHVPPKVSHITFAHEVGHNFGSPHDSGTECTPGESKNLGQKENGNYIMYARATSGDKLNNNKFSLCSIRNISQVLEKKRNNCFVESGQPICGNGMVEQGEECDCGYSDQCKDECCFDANQPEGRKCKLKPGKQCSTVCIQVKV 相同性解析による交差性が予測される生物種 |
| Full Name | ADAM metallopeptidase domain 10 |
| Calculated molecular weight | 748 aa, 84 kDa |
| Observed molecular weight | 60-70 kDa, 90 kDa |
| GenBank accession number | BC126253 |
| Gene Symbol | ADAM10 |
| Gene ID (NCBI) | 102 |
| RRID | AB_2880291 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14672 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADAM10 antibody 25900-1-AP | Download protocol |
| WB protocol for ADAM10 antibody 25900-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Social memory maintenance relies on social interaction-induced proteolytic products of neuroligin 1. | ||
Cell Elucidating pathway-selective biased CCKBR agonism for Alzheimer's disease treatment. | ||
Nat Commun ADAM9 promotes type I interferon-mediated innate immunity during encephalomyocarditis virus infection | ||
J Adv Res Luteolin as a novel therapeutic for diabetic kidney disease: Targeting the ADAM10-TREM2 pathway. | ||
Neuron Maternal-fetal type I interferon signaling drives TREM2 dysregulation and synaptic dysfunction in neurodevelopmental disorders. | ||