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ACTN4 Polyclonal antibody

ACTN4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 19096-1-AP


ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

Tested Applications

Positive WB detected inHEK-293T cells, mouse liver tissue, HeLa cells, Jurkat cells, C2C12 cells, NIH/3T3 cells, mouse skeletal muscle, rat skeletal muscle
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19096-1-AP targets ACTN4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTN4 fusion protein Ag13546 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 4
Calculated molecular weight 105 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC005033
Gene symbol ACTN4
Gene ID (NCBI) 81
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)


Product Specific Protocols
WB protocol for ACTN4 antibody 19096-1-APDownload protocol
IHC protocol for ACTN4 antibody 19096-1-APDownload protocol
IF protocol for ACTN4 antibody 19096-1-APDownload protocol
IP protocol for ACTN4 antibody 19096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Thorac Oncol

Alpha-actinin 4 is Associated with Cancer Cell Motility and is a Potential Biomarker in Non-Small-Cell Lung Cancer.

Authors - Ming-Chuan Wang


MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.

Authors - Agarwal N N

Cancer Immunol Immunother

Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.

Authors - Xiuxing Chen

Sci Rep

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.

Authors - Hongfei Ge

J Cell Sci

GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region

Authors - Paroma Chatterjee

Amino Acids

Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.

Authors - Chen Bai