Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of mouse skeletal muscle using 24378-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24378-1-AP (ACTN3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 24378-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24378-1-AP (ACTN3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 24378-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 24378-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24378-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24378-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse skeletal muscle using 24378-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (24378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse skeletal muscle using 24378-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (24378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ACTN3 Polyclonal antibody

ACTN3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 24378-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

actinin, alpha 3, ACTN 3, Alpha actinin 3, Alpha-actinin skeletal muscle isoform 3, Alpha-actinin-3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected in	mouse skeletal muscle tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse skeletal muscle tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

24378-1-AP targets ACTN3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19479 Product name: Recombinant human ACTN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 577-624 aa of BC099647 Sequence: RERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKL 相同性解析による交差性が予測される生物種
Full Name	actinin, alpha 3
Calculated molecular weight	901 aa, 103 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC099647
Gene Symbol	ACTN3
Gene ID (NCBI)	89
RRID	AB_2879515
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q08043
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for ACTN3 antibody 24378-1-AP	Download protocol
IHC protocol for ACTN3 antibody 24378-1-AP	Download protocol
WB protocol for ACTN3 antibody 24378-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Dokl Biochem Biophys Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains? Authors - I O Petrova View Article
mouse	WB	Cell Metab Muscle-derived small extracellular vesicles induce liver fibrosis during overtraining Authors - Ya Liu View Article
mouse	WB	FASEB J Overexpression of enhanced yellow fluorescent protein fused with Channelrhodopsin-2 causes contractile dysfunction in skeletal muscle Authors - Syeda N Lamia View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse skeletal muscle using 24378-1-AP

WB analysis using 24378-1-AP

IHC staining of mouse skeletal muscle using 24378-1-AP