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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    WB analysis of human testis using 13537-1-AP (same clone as 13537-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13537-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    WB analysis of mouse testis using 13537-1-AP (same clone as 13537-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13537-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    WB analysis of human liver using 13537-1-AP (same clone as 13537-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    IHC staining of human prostate cancer using 13537-1-AP (same clone as 13537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13537-1-AP (ACTL7B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    IHC staining of human prostate cancer using 13537-1-AP (same clone as 13537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13537-1-AP (ACTL7B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13537-1-PBS in a different storage buffer formulation.

ACTL7B Polyclonal antibody, PBS Only

ACTL7B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13537-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13537-1-PBS targets ACTL7B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4460

Product name: Recombinant human ACTL7B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-415 aa of BC033789

Sequence: MATRNSPMPLGTAQGDPGEAGTRPGPDASLRDTGAATQLKMKPRKVHKIKAVIIDLGSQYCKCGYAGEPRPTYFISSTVGKRCPEAADAGDTRKWTLVGHELLNTEAPLKLVNPLKHGIVVDWDCVQDIWEYIFRTAMKILPEEHAVLVSDPPLSPSSNREKYAELMFETFGIPAMHVTSQSLLSIYSYGKTSGLVVESGHGVSHVVPISEGDVLPGLTSRADYAGGDLTNYLMQLLNEAGHAFTDDHLHIIEHIKKKCCYAAFLPEEELGLVPEELRVDYELPDGKLITIGQERFRCSEMLFQPSLAGSTQPGLPELTAACLGRCQDTGFKEEMAANVLLCGGCTMLDGFPERFQRELSLLCPGDSPAVAAAPERKTSVWTGGSILASLQAFQQLWVSKEEFEERGSVAIYSKC

相同性解析による交差性が予測される生物種
Full Name actin-like 7B
Calculated molecular weight 415 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC033789
Gene Symbol ACTL7B
Gene ID (NCBI) 10880
RRIDAB_2223776
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y614
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACTL7B is also named as actin-like-7-beta. The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. The ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known. Diseases associated with ACTL7B include Dysautonomia and Hypertensive Nephropathy. An important paralog of this gene is ACTL7A. ACTL7B can be detected as 45 kDa.

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