Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human colon tissue, human skin cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 1 publications below |
Product Information
11227-1-AP targets Gamma Actin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1728 Product name: Recombinant human ACTG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-375 aa of BC009848 Sequence: MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 相同性解析による交差性が予測される生物種 |
| Full Name | actin, gamma 1 |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 42-45 kDa |
| GenBank accession number | BC009848 |
| Gene Symbol | Gamma Actin |
| Gene ID (NCBI) | 71 |
| RRID | AB_2223507 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P63261 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Gamma Actin antibody 11227-1-AP | Download protocol |
| WB protocol for Gamma Actin antibody 11227-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart | ||
Front Mol Neurosci F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model. | ||
Front Neurosci Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic rats | ||
FEBS J Icariin ameliorates glycolytic dysfunction in Alzheimer's disease models by activating the Wnt/β-catenin signaling pathway | ||
Ultrasound Q Ultrasound Microbubble-Stimulated miR-145-5p Inhibits Malignant Behaviors of Breast Cancer Cells by Targeting ACTG1 | ||
Cells Interaction of Receptor-Binding Domain of the SARS-CoV-2 Omicron Variant with hACE2 and Actin |
