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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha cardiac muscle actin specific Polyclonal ant (17522-1-AP)

    WB analysis using 17522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17522-1-AP (Actin-cardiac-muscle-1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Alpha cardiac muscle actin specific Polyclonal antibody

Alpha cardiac muscle actin specific Polyclonal Antibody for WB, ELISA
Cat No. 17522-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Actin-cardiac-muscle-1-Specific, ACTC1, Alpha actin, ACTC, ACTC 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17522-1-AP targets Alpha cardiac muscle actin specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 42 kDa
Observed molecular weight42 kDa
GenBank accession numberNM_005159
Gene Symbol ACTC1
Gene ID (NCBI) 70
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68032
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.

Protocols

Product Specific Protocols
WB protocol for Alpha cardiac muscle actin specific antibody 17522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols