Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-FroIF/ICCFC (Intra)

WB analysis using 14395-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14395-1-AP
WB result of smooth muscle actin antibody (14395-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin transfected A549 cells.
WB analysis using 14395-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 14395-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 14395-1-AP
IP result of anti-smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.
IHC staining of human appendicitis using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).
IHC staining of mouse colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 40x lens).
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of stromal tumor using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens).
IHC staining of stromal tumor using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens).
IHC staining of human hysteromyoma using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hysteromyoma using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human colon using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Alpha smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human colon using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Alpha smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse small intestine using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha smooth muscle actin antibody (14395-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse colon using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of C2C12 using 14395-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of H9C2 using 14395-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of C2C12 using 14395-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of C2C12 using 14395-1-AP
1x10^6 C2C12 cells were intracellularly stained with 0.4 ug smooth muscle actin Polyclonal antibody (14395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

Alpha smooth muscle actin Polyclonal antibody

Alpha smooth muscle actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Cat No. 14395-1-AP

Publications(2319)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, competitive binding assay, Western Blot

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2319)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse colon tissue, human placenta tissue, A549 cells, HUVEC cells, rat colon tissue, mouse heart tissue, rat heart tissue, mouse liver tissue, mouse kidney tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human appendicitis tissue, human colon tissue, human heart tissue, human hysteromyoma tissue, human liver cancer tissue, mouse colon tissue, stromal tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue, mouse small intestine tissue, human colon tissue
Positive IF-Fro detected in	mouse colon tissue
Positive IF/ICC detected in	C2C12 cells, H9C2 cells
Positive FC (Intra) detected in	C2C12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:4000-1:16000
Immunofluorescence (IF)-P	IF-P : 1:1000-1:4000
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:800-1:3200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 1359 publications below
IHC	See 622 publications below
IF	See 864 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

14395-1-AP targets Alpha smooth muscle actin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, competitive binding assay, Western Blot applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, monkey, chicken, zebrafish, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5886 Product name: Recombinant human ACTA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC017554 Sequence: MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF 相同性解析による交差性が予測される生物種
Full Name	actin, alpha 2, smooth muscle, aorta
Calculated molecular weight	377 aa, 42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC017554
Gene Symbol	Alpha smooth muscle actin
Gene ID (NCBI)	59
RRID	AB_2223009
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62736
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Protocols

Product Specific Protocols
FC protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
IF protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
IHC protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
IP protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
WB protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Signal Transduct Target Ther Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis Authors - Xiaofei Huang View Article
human	IF	Cancer Cell Pan-cancer human brain metastases atlas at single-cell resolution Authors - Xudong Xing View Article
		Cell Meningeal blood vessel blockage enhances anti-glioblastoma immunity. Authors - Yixin Gao View Article
mouse,human	IHC,IF	Mol Cancer CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment Authors - Zongfu Pan View Article
	WB	Mol Cancer Transcriptional landscape and predictive potential of long noncoding RNAs in peritoneal recurrence of gastric cancer Authors - Xiao-Xia Cai View Article
		Mol Cancer Modulation of tumor-derived exosomes and reprogramming of cancer-associated fibroblasts for colorectal cancer therapy. Authors - Susu Han View Article

Validation Data Gallery

WB analysis using 14395-1-AP

WB analysis of A549 using 14395-1-AP

WB analysis using 14395-1-AP

WB analysis of human placenta using 14395-1-AP

IP experiment of mouse liver using 14395-1-AP

IHC staining of human appendicitis using 14395-1-AP

IHC staining of human liver cancer using 14395-1-AP

IHC staining of human colon using 14395-1-AP

IHC staining of human liver cancer using 14395-1-AP

IHC staining of human heart using 14395-1-AP

IHC staining of mouse colon using 14395-1-AP

IHC staining of mouse colon using 14395-1-AP

IHC staining of human appendicitis using 14395-1-AP

IHC staining of human heart using 14395-1-AP

IHC staining of human liver cancer using 14395-1-AP

IHC staining of human liver cancer using 14395-1-AP

IHC staining of human colon using 14395-1-AP

IHC staining of stromal tumor using 14395-1-AP

IHC staining of stromal tumor using 14395-1-AP

IHC staining of human hysteromyoma using 14395-1-AP

IHC staining of human hysteromyoma using 14395-1-AP

IF Staining of human colon using 14395-1-AP

IF Staining of human colon using 14395-1-AP

IF Staining of mouse small intestine using 14395-1-AP

IF Staining of mouse heart using 14395-1-AP

IF Staining of mouse colon using 14395-1-AP

IF Staining of C2C12 using 14395-1-AP

IF Staining of H9C2 using 14395-1-AP

IF Staining of C2C12 using 14395-1-AP

FC experiment of C2C12 using 14395-1-AP

Alpha smooth muscle actin Polyclonal antibody

Alpha smooth muscle actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis

Pan-cancer human brain metastases atlas at single-cell resolution

Meningeal blood vessel blockage enhances anti-glioblastoma immunity.

CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment

Transcriptional landscape and predictive potential of long noncoding RNAs in peritoneal recurrence of gastric cancer

Modulation of tumor-derived exosomes and reprogramming of cancer-associated fibroblasts for colorectal cancer therapy.

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