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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSS2 Recombinant antibody (84774-7-RR)

    WB analysis using 84774-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84774-7-RR (ACSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Caco-2 cells using ACSS2 Recombinant antibody (84774-7-RR)

    FC experiment of Caco-2 using 84774-7-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of HepG2 cells using ACSS2 Recombinant antibody (84774-7-RR)

    FC experiment of HepG2 using 84774-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84774-7-RR

    Affinity and Kinetic Characterization of 84774-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84774-7-RR against Human ACSS2 were performed. The affinity constant is 91.2 nM.

ACSS2 Recombinant monoclonal antibody

ACSS2 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84774-7-RR
Clone No.242253C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

242253C6, ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Caco-2 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells, Caco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84774-7-RR targets ACSS2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9121

Product name: Recombinant human ACSS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 185-534 aa of BC010141

Sequence: VFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDVNRLWSIVDKYKVTKFYTAPTAIRLLMKFGDEPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGAQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPKLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDMSTVADPSVISHLFSHRCLTIQ

相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase short-chain family member 2
Calculated molecular weight 701 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC010141
Gene Symbol ACSS2
Gene ID (NCBI) 55902
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NR19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714).

Protocols

Product Specific Protocols
FC protocol for ACSS2 antibody 84774-7-RRDownload protocol
WB protocol for ACSS2 antibody 84774-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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