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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSM5 Recombinant antibody (84444-1-RR)

    WB analysis using 84444-1-RR (same clone as 84444-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84444-1-RR (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84444-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ACSM5 Recombinant antibody (84444-1-RR)

    IHC staining of mouse liver using 84444-1-RR (same clone as 84444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84444-1-RR (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84444-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using ACSM5 Recombinant antibody (84444-1-RR)

    FC experiment of HepG2 using 84444-1-RR (same clone as 84444-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSM5 Recombinant antibody (84444-1-RR, Clone:241400E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84444-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84444-1-RR

    Affinity and Kinetic Characterization of 84444-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84444-1-RR against Human ACSM5 were performed. The affinity constant is below 1 pM.

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ACSM5 Recombinant antibody, PBS Only

ACSM5 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84444-1-PBS
Clone No.241400E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

241400E7, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84444-1-PBS targets ACSM5 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACSM5 fusion protein Ag9373 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase medium-chain family member 5
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC016703
Gene Symbol ACSM5
Gene ID (NCBI) 54988
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6NUN0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.