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Validation Data Gallery

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  • Western Blot (WB) analysis of 4T1 cells using ACSM5 Monoclonal antibody (67334-1-Ig)

    WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    WB analysis of rat liver using 67334-1-Ig (same clone as 67334-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using ACSM5 Monoclonal antibody (67334-1-Ig)

    WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-231 cells using ACSM5 Monoclonal antibody (67334-1-Ig)

    WB analysis of MDA-MB-231 using 67334-1-Ig (same clone as 67334-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACSM5 Monoclonal antibody (67334-1-Ig)

    IF Staining of HepG2 using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM5 antibody (67334-1-Ig, Clone: 2D5D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

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ACSM5 Monoclonal antibody, PBS Only

ACSM5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67334-1-PBS
Clone No.2D5D6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ACSM5, FLJ20581, MACS3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67334-1-PBS targets ACSM5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACSM5 fusion protein Ag10056 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase medium-chain family member 5
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC016703
Gene Symbol ACSM5
Gene ID (NCBI) 54988
RRIDAB_2882592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6NUN0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.