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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ACSM3 Polyclonal antibody (10168-2-AP)

    WB analysis of HEK-293 using 10168-2-AP (same clone as 10168-2-PBS)

    WB result of ACSM3 antibody (10168-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSM3 transfected HEK-293 cells. This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACSM3 Polyclonal antibody (10168-2-AP)

    WB analysis using 10168-2-AP (same clone as 10168-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10168-2-AP (ACSM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ACSM3 Polyclonal antibody (10168-2-AP)

    IHC staining of human colon cancer using 10168-2-AP (same clone as 10168-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10168-2-AP (ACSM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACSM3 Polyclonal antibody (10168-2-AP)

    IHC staining of human prostate cancer using 10168-2-AP (same clone as 10168-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACSM3 Polyclonal antibody (10168-2-AP)

    IHC staining of human prostate cancer using 10168-2-AP (same clone as 10168-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACSM3 Polyclonal antibody (10168-2-AP)

    IF Staining of HepG2 using 10168-2-AP (same clone as 10168-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM3 antibody (10168-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10168-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ACSM3 Polyclonal antibody, PBS Only

ACSM3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10168-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10168-2-PBS targets ACSM3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0224

Product name: Recombinant human ACSM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC002790

Sequence: MLARVTRKMLRHAKCFQRLAIFGSVRALHKDNRTATPQNFSNYESMKQDFKLGIPEYFNFAKDVLDQWTDKEKAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSENSREGWGNLKELMKHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHHLPRFEPTSILQTLSKYPI

相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase medium-chain family member 3
Calculated molecular weight 49 kDa, 66 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC002790
Gene Symbol ACSM3
Gene ID (NCBI) 6296
RRIDAB_2222699
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53FZ2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SA hypertension-associated rat homolog (SAH, synonym: SA) is expressed at 10-fold greater levels in the kidney of the spontaneously hypertensive rat than in the corresponding wild-type strain. The gene is linked to blood pressure levels in a number of crosses involving the spontaneously hypertensive rat and other strains of genetically hypertensive rats. Human SAH gene polymorphism may be associated with the renal prognosis of immunoglobulin A nephropathy through its effect on blood pressure. Furthermore, variation in SAH has a role in predisposition.

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