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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSL6 Polyclonal antibody (13847-1-AP)

    WB analysis using 13847-1-AP (same clone as 13847-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13847-1-AP (ACSL6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13847-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ACSL6 Polyclonal antibody (13847-1-AP)

    IP experiment of mouse brain using 13847-1-AP (same clone as 13847-1-PBS)

    IP result of anti-ACSL6 (IP:13847-1-AP, 4ug; Detection:13847-1-AP 1:2000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 13847-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using ACSL6 Polyclonal antibody (13847-1-AP)

    IHC staining of human urothelial carcinoma using 13847-1-AP (same clone as 13847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13847-1-AP (ACSL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13847-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ACSL6 Polyclonal antibody (13847-1-AP)

    IHC staining of human pancreas cancer using 13847-1-AP (same clone as 13847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13847-1-AP (ACSL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13847-1-PBS in a different storage buffer formulation.

ACSL6 Polyclonal antibody, PBS Only

ACSL6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13847-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ACSL 6, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.3, FACL6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13847-1-PBS targets ACSL6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4817

Product name: Recombinant human ACSL6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 48-349 aa of BC047453

Sequence: THYYDDARTMYQVFRRGLSISGNGPCLGFRKPKQPYQWLSYQEVADRAEFLGSGLLQHNCKACTDQFIGVFAQNRPEWIIVELACYTYSMVVVPLYDTLGPGAIRYIINTADISTVIVDKPQKAVLLLEHVERKETPGLKLIILMDPFEEALKERGQKCGVVIKSMQAVEDCGQANHQAPVPPQPDDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTEGDIRLLSDDMKALCPTIFPVVPRLLNRMYDKIFSQANTPLKRWLLEFAAKRKQAEVRSGIIRNDSIWDELFFNKIQASL

相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase long-chain family member 6
Calculated molecular weight 78 kDa
Observed molecular weight74 kDa
GenBank accession numberBC047453
Gene Symbol ACSL6
Gene ID (NCBI) 23305
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKU0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACSL6 (Acyl-CoA Synthetase Long Chain Family Member 6) is a long-chain lipoyl-coenzyme A synthetase that is mainly responsible for catalyzing the binding of long-chain fatty acids to coenzyme A to form acyl-coenzyme A (Acyl-CoA). ACSL6 is involved in fatty acid metabolic transporter and the synthesis of cell membrane phospholipids, which affects the cell structure and function. ACSL6 plays an extremely important role in the pathogenesis of cancers such as hepatocellular carcinoma, lung cancer and neuroblastoma, suggesting that it may serve as a biomarker and therapeutic target for cancer therapy.

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