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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using ACSL5 Recombinant monoclonal antibody (86982-1-RR)

    WB analysis of rat liver using 86982-1-RR (same clone as 86982-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 86982-1-RR (ACSL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ACSL5 Recombinant monoclonal antibody (86982-1-RR)

    WB analysis of mouse liver using 86982-1-RR (same clone as 86982-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 86982-1-RR (ACSL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86982-1-RR

    Affinity and Kinetic Characterization of 86982-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86982-1-RR against Human ACSL5 were performed. The affinity constant is below 1 pM.

ACSL5 Recombinant monoclonal antibody, PBS Only

ACSL5 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86982-1-PBS
Clone No.252098G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

ACSL 5, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.3, FACL5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86982-1-PBS targets ACSL5 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8258

Product name: Recombinant human ACSL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 388-739 aa of BC007985

Sequence: DIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase long-chain family member 5
Calculated molecular weight 76 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC007985
Gene Symbol ACSL5
Gene ID (NCBI) 51703
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9ULC5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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