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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using ACSL5 Recombinant monoclonal antibody (86982-1-RR)

    WB analysis of rat liver using 86982-1-RR (same clone as 86982-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 86982-1-RR (ACSL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ACSL5 Recombinant monoclonal antibody (86982-1-RR)

    WB analysis of mouse liver using 86982-1-RR (same clone as 86982-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 86982-1-RR (ACSL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACSL5 Recombinant monoclonal antibody (86982-1-RR)

    IHC staining of human liver using 86982-1-RR (same clone as 86982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86982-1-RR (ACSL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACSL5 Recombinant monoclonal antibody (86982-1-RR)

    IHC staining of human liver using 86982-1-RR (same clone as 86982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86982-1-RR (ACSL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86982-1-RR

    Affinity and Kinetic Characterization of 86982-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86982-1-RR against Human ACSL5 were performed. The affinity constant is below 1 pM.

ACSL5 Recombinant monoclonal antibody, PBS Only

ACSL5 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86982-1-PBS
Clone No.252098G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ACSL 5, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.3, FACL5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86982-1-PBS targets ACSL5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8258

Product name: Recombinant human ACSL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 388-739 aa of BC007985

Sequence: DIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase long-chain family member 5
Calculated molecular weight 76 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC007985
Gene Symbol ACSL5
Gene ID (NCBI) 51703
RRIDAB_3745254
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9ULC5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACSL5 (acyl-CoA synthetase long-chain family member 5) is a gene encoding an enzyme that activates long-chain fatty acids into acyl-CoA esters, thereby directing them toward lipid synthesis or β-oxidation. It is predominantly expressed in the small intestine, uterus, and spleen, with minimal presence in normal brain tissue. ACSL5 has gained clinical attention for its marked upregulation in malignant gliomas, where it promotes fatty-acid-driven tumor growth, and its mutations are linked to congenital diarrhea (DIAR13). Additionally, its expression levels are being explored as prognostic markers in colorectal cancer and as modulators of apoptotic signaling.

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