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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ACSL3 Polyclonal antibody (20710-1-AP)

    WB analysis of HEK-293 using 20710-1-AP (same clone as 20710-1-PBS)

    WB result of ACSL3 antibody (20710-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL3 transfected HEK-293 cells. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACSL3 Polyclonal antibody (20710-1-AP)

    WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACSL3 Polyclonal antibody (20710-1-AP)

    WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ACSL3 Polyclonal antibody (20710-1-AP)

    IHC staining of human stomach cancer using 20710-1-AP (same clone as 20710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20710-1-AP (ACSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ACSL3 Polyclonal antibody, PBS Only

ACSL3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20710-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

ACS3, ACSL 3, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20710-1-PBS targets ACSL3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase long-chain family member 3
Calculated molecular weight 80 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberNM_004457
Gene Symbol ACSL3
Gene ID (NCBI) 2181
RRIDAB_10697670
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95573
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.

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