Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse heart tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
25484-1-AP targets ACSF3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21979 Product name: Recombinant human ACSF3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 226-576 aa of BC028399 Sequence: LVHKWAWTKDDVILHVLPLHHVHGVVNALLCPLWVGATCVMMPEFSPQQVWEKFLSSETPRINVFMAVPTIYTKLMEYYDRHFTQPHAQDFLRAVCEEKIRLMVSGSAALPLPVLEKWKNITGHTLLERYGMTEIGMALSGPLTTAMRLPGSVGTPLPGVQVRIVSENPQREACSYTIHAEGDERGTKVTPGFEEKEGELLVRGPSVFREYWNKPEETKSAFTLDGWFKTGDTVVFKDGQYWIRGRTSVDIIKTGGYKVSALEVEWHLLAHPSITDVAVIGVPDMTWGQRVTAVVTLREGHSLSHRELKEWARNVLAPYAVPSELVLVEEIPRNQMGKIDKKALIRHFHPS 相同性解析による交差性が予測される生物種 |
| Full Name | acyl-CoA synthetase family member 3 |
| Calculated molecular weight | 576 aa, 64 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC028399 |
| Gene Symbol | ACSF3 |
| Gene ID (NCBI) | 197322 |
| RRID | AB_2880099 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q4G176 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ACSF3 antibody 25484-1-AP | Download protocol |
| WB protocol for ACSF3 antibody 25484-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Genom An ancient regulatory variant of ACSF3 influences the coevolution of increased human height and basal metabolic rate via metabolic homeostasis | ||
Bioconjug Chem Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.
| ||
Sci Transl Med Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin |