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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACSBG1 Polyclonal antibody

ACSBG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16077-1-AP

Synonyms

ACSBG1, BG1, BGM, GR LACS, hBG1, hsBG, hsBGM, KIAA0631, Lipodisin, LPD

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 16077-1-AP

    WB analysis of mouse testis using 16077-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16077-1-AP (ACSBG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat testis using 16077-1-AP

    WB analysis of rat testis using 16077-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 16077-1-AP (ACSBG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 16077-1-AP

    IHC staining of mouse testis using 16077-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16077-1-AP (ACSBG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 16077-1-AP

    IHC staining of mouse testis using 16077-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16077-1-AP (ACSBG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16077-1-AP targets ACSBG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSBG1 fusion protein Ag9043 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase bubblegum family member 1
Calculated molecular weight 724 aa, 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC009289
Gene symbol ACSBG1
Gene ID (NCBI) 23205
RRIDAB_2878214
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACSBG1 antibody 16077-1-APDownload protocol
IHC protocol for ACSBG1 antibody 16077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Endocrinology

mTOR regulates mineralocorticoid receptor transcriptional activity by ULK1- dependent and independent mechanisms

Authors - Yusuf Ali
View Article