Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells, K-562 cells, RAW 264.7 cells, NIH/3T3 cells, PC-12 cells |
| Positive IF/ICC detected in | 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86817-1-RR targets ACP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag17539 Product name: Recombinant human ACP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 15-102 aa of BC007422 Sequence: GNICRSPIAEAVFRKLVTDQNISENWVIDSGAVSDWNVGRSPDPRAVSCLRNHGIHTAHKARQITKEDFATFDYILCMDESNLRDLNR 相同性解析による交差性が予測される生物種 |
| Full Name | acid phosphatase 1, soluble |
| Calculated molecular weight | 158 aa, 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC007422 |
| Gene Symbol | ACP1 |
| Gene ID (NCBI) | 52 |
| RRID | AB_3745140 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P24666 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Acid phosphatase 1 (ACP1) is a low-molecular-weight phosphotyrosine protein phosphatase known to participate in various cellular processes, including signal transduction, proliferation, and metabolism. It functions by dephosphorylating tyrosine-phosphorylated proteins, thereby modulating receptor-mediated signaling pathways.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACP1 antibody 86817-1-RR | Download protocol |
| WB protocol for ACP1 antibody 86817-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |