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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOX3 Recombinant monoclonal antibody (84027-4-RR)

    WB analysis using 84027-4-RR (same clone as 84027-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84027-4-RR (ACOX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84027-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using ACOX3 Recombinant monoclonal antibody (84027-4-RR)

    FC experiment of A431 using 84027-4-RR (same clone as 84027-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX3 Recombinant antibody (84027-4-RR, Clone:241172G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84027-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84027-4-RR

    Affinity and Kinetic Characterization of 84027-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84027-4-RR against Human ACOX3 were performed. The affinity constant is 1.05 nM.

ACOX3 Recombinant monoclonal antibody, PBS Only

ACOX3 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84027-4-PBS
Clone No.241172G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

241172G8, ACOX 3, Branched-chain acyl-CoA oxidase, BRCACox, BRCOX

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84027-4-PBS targets ACOX3 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag10553

Product name: Recombinant human ACOX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 332-624 aa of BC017053

Sequence: TRRQFGPTEEEEIPVLEYPMQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREIHALASASKPLASWTTQQGIQECREACGGHGYLAMNRLGVLRDDNDPNCTYEGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLDAYPGILDQKFEVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKCQVSHGRPLALAFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSLSRHAALLYRAERRCSCPGRRDRSS

相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A oxidase 3, pristanoyl
Calculated molecular weight 624 aa, 70 kDa
Observed molecular weight70-78 kDa
GenBank accession numberBC017053
Gene Symbol ACOX3
Gene ID (NCBI) 8310
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO15254
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3) is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes.

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