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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOT11 Polyclonal antibody (10776-1-AP)

    WB analysis using 10776-1-AP (same clone as 10776-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10776-1-AP (ACOT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACOT11 Polyclonal antibody (10776-1-AP)

    IHC staining of human prostate cancer using 10776-1-AP (same clone as 10776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10776-1-AP (ACOT11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACOT11 Polyclonal antibody (10776-1-AP)

    IHC staining of human prostate cancer using 10776-1-AP (same clone as 10776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10776-1-AP (ACOT11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ACOT11 Polyclonal antibody (10776-1-AP)

    IF Staining of A431 using 10776-1-AP (same clone as 10776-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10776-1-AP (ACOT11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ACOT11 Polyclonal antibody (10776-1-AP)

    IF Staining of U2OS using 10776-1-AP (same clone as 10776-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ACOT11 antibody (10776-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

ACOT11 Polyclonal antibody, PBS Only

ACOT11 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10776-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10776-1-PBS targets ACOT11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1176

Product name: Recombinant human ACOT11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-221 aa of BC001517

Sequence: MADGEGYRNPTEVQMSQLVLPCHTNQRGELSVGQLLKWIDTTACLSAERHAGCPCVTASMDDIYFEHTISVGQVVNIKAKVNRAFNSSMEVGIQVASEDLCSEKQWNVCKALATFVARREITKVKLKQITPRTEEEKMEHSVAAERRRMRLVYADTIKDLLANCAIQGDLESRDCSRMVPAEKTRVESVELVLPPHANHQGNTFGGQIMAWMENVATIAAR

相同性解析による交差性が予測される生物種
Full Name acyl-CoA thioesterase 11
Calculated molecular weight68.4 kDa
Observed molecular weight68-70 kDa
GenBank accession numberBC001517
Gene Symbol ACOT11
Gene ID (NCBI) 26027
RRIDAB_2257798
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXI4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACOT11(Acyl-coenzyme A thioesterase 11) is also named as BFIT, KIAA0707, THEA.BFIT is mapped to syntenic regions of chromosomes 1 (human) and 4 (mouse) associated with body fatness and diet-induced obesity, potentially linking a deficit of BFIT activity with exacerbation of these traits and supports the transition of this tissue towards increased metabolic activity, probably through alteration of intracellular fatty acyl-CoA concentration(PMID:11696000).

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