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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ACLY Monoclonal antibody (67166-1-Ig)

    WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ACLY Monoclonal antibody (67166-1-Ig)

    WB analysis of HSC-T6 using 67166-1-Ig (same clone as 67166-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ACLY Monoclonal antibody (67166-1-Ig)

    WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ACLY Monoclonal antibody (67166-1-Ig)

    WB analysis of MCF-7 using 67166-1-Ig (same clone as 67166-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACLY Monoclonal antibody (67166-1-Ig)

    IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACLY Monoclonal antibody (67166-1-Ig)

    IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ACLY Monoclonal antibody (67166-1-Ig)

    IF Staining of A549 using 67166-1-Ig (same clone as 67166-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACLY antibody (67166-1-Ig, Clone: 1E10D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ACLY Monoclonal antibody, PBS Only

ACLY Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67166-1-PBS
Clone No.1E10D5

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ATP citrate lyase, 1E10D5, ACL, ATP-citrate, ATP-citrate synthase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67166-1-PBS targets ACLY in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7709

Product name: Recombinant human ACLY protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 751-1101 aa of BC006195

Sequence: MFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

相同性解析による交差性が予測される生物種
Full Name ATP citrate lyase
Calculated molecular weight 121 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC006195
Gene Symbol ACLY
Gene ID (NCBI) 47
RRIDAB_2882462
Conjugate Unconjugated
Form
FormLiquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP53396
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

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