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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of A549 using 14304-1-AP (same clone as 14304-1-PBS)

    WB result of ACK1 antibody (14304-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACK1 transfected A549 cells. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of SH-SY5Y using 14304-1-AP (same clone as 14304-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of HT-1080 using 14304-1-AP (same clone as 14304-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of A431 using 14304-1-AP (same clone as 14304-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of mouse brain using 14304-1-AP (same clone as 14304-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of HeLa using 14304-1-AP (same clone as 14304-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of HepG2 using 14304-1-AP (same clone as 14304-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACK1 Polyclonal antibody (14304-1-AP)

    IHC staining of mouse kidney using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14304-1-AP (ACK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACK1 Polyclonal antibody (14304-1-AP)

    IHC staining of mouse kidney using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14304-1-AP (ACK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ACK1 Polyclonal antibody, PBS Only

ACK1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14304-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

TNK2, ACK, ACK 1, ACK-1, Activated CDC42 kinase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14304-1-PBS targets ACK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5419

Product name: Recombinant human ACK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-385 aa of BC028164

Sequence: MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKALCKRKSWMSKVFSGKRLEAEFPPHHSQSTFRKTSPAPGGPAGEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRLRKHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFL

相同性解析による交差性が予測される生物種
Full Name tyrosine kinase, non-receptor, 2
Calculated molecular weight 115 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC028164
Gene Symbol TNK2
Gene ID (NCBI) 10188
RRIDAB_11041713
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07912
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TNK2, also named ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, and cell proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.

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