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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACBD3 Polyclonal antibody

ACBD3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14096-1-AP

Synonyms

ACBD3, GCP60, GOCAP1, Golgi phosphoprotein 1, Golgi resident protein GCP60, GOLPH1, PAP7

Validation Data Gallery

Applications:
  • WB analysis using 14096-1-AP

    WB analysis using 14096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14096-1-AP (ACBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 14096-1-AP

    WB analysis of mouse testis using 14096-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14096-1-AP (ACBD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse ovary using 14096-1-AP

    WB analysis of mouse ovary using 14096-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14096-1-AP (ACBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 14096-1-AP

    IHC staining of mouse testis using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 14096-1-AP

    IHC staining of mouse testis using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14096-1-AP

    IF Staining of HeLa using 14096-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14096-1-AP (ACBD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, mouse ovary tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14096-1-AP targets ACBD3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACBD3 fusion protein Ag5228 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A binding domain containing 3
Calculated molecular weight 528 aa, 61 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC060792
Gene symbol ACBD3
Gene ID (NCBI) 64746
RRIDAB_2220062
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACBD3 antibody 14096-1-APDownload protocol
IHC protocol for ACBD3 antibody 14096-1-APDownload protocol
IF protocol for ACBD3 antibody 14096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols