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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACAT1 Monoclonal antibody (60485-4-Ig)

    WB analysis using 60485-4-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60485-4-Ig (ACAT1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ACAT1 Monoclonal antibody (60485-4-Ig)

    WB analysis using 60485-4-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60485-4-Ig (ACAT1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

ACAT1 Monoclonal antibody

ACAT1 Monoclonal Antibody for WB, ELISA
Cat No. 60485-4-Ig
Clone No.1F3H1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, 4T1 cells, rabbit brain tissue, pig brain tissue, A549 cells, HepG2 cells, MJ cells, Karpas-422 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60485-4-Ig targets ACAT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9219

Product name: Recombinant human ACAT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-162 aa of BC010942

Sequence: MAVLPALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQIKQETGSLAKICCHVRR

相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A acetyltransferase 1
Calculated molecular weight 45 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC010942
Gene Symbol ACAT1
Gene ID (NCBI) 38
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP24752
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

Protocols

Product Specific Protocols
WB protocol for ACAT1 antibody 60485-4-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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