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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis of MCF-7 using 16215-1-AP (same clone as 16215-1-PBS)

    WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis using 16215-1-AP (same clone as 16215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis of mouse heart using 16215-1-AP (same clone as 16215-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis of rat skeletal muscle using 16215-1-AP (same clone as 16215-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis of human skeletal muscle using 16215-1-AP (same clone as 16215-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    IP experiment of mouse heart using 16215-1-AP (same clone as 16215-1-PBS)

    IP result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon using ACAT1 Polyclonal antibody (16215-1-AP)

    IHC staining of human colon using 16215-1-AP (same clone as 16215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ACAT1 Polyclonal antibody (16215-1-AP)

    IF Staining of HeLa using 16215-1-AP (same clone as 16215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ACAT1 antibody (16215-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ACAT1 Polyclonal antibody (16215-1-AP)

    FC experiment of HeLa using 16215-1-AP (same clone as 16215-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACAT1 (16215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ACAT1 Polyclonal antibody, PBS Only

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 16215-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

MAT, EC:2.3.1.9, Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, Acetoacetyl CoA thiolase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16215-1-PBS targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAT1 fusion protein Ag9215 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A acetyltransferase 1
Calculated molecular weight 45 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC010942
Gene Symbol ACAT1
Gene ID (NCBI) 38
RRIDAB_2220210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24752
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.