WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

mouse heart tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

IP result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ACAT1 antibody (16215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACAT1 (16215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.