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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ACAP3 Polyclonal antibody (17570-1-AP)

    WB analysis of Jurkat using 17570-1-AP (same clone as 17570-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17570-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using ACAP3 Polyclonal antibody (17570-1-AP)

    WB analysis of SH-SY5Y using 17570-1-AP (same clone as 17570-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17570-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using ACAP3 Polyclonal antibody (17570-1-AP)

    IF Staining of SH-SY5Y using 17570-1-AP (same clone as 17570-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 17570-1-AP (ACAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17570-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17570-1-PBS targets ACAP3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAP3 fusion protein Ag11777 相同性解析による交差性が予測される生物種
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 3
Calculated molecular weight 834 aa, 92 kDa
Observed molecular weight85kda, 92 kDa
GenBank accession numberBC047001
Gene Symbol ACAP3
Gene ID (NCBI) 116983
RRIDAB_2257668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96P50
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACAP3 (Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 3) , also known as CENTB5, is a protein-coding gene that belongs to the ACAP family of GTPase-activating proteins (GAPs). ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer, ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer (PMID: 28919417),