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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ACAP1 Recombinant antibody (82967-1-RR)

    WB analysis of Jurkat using 82967-1-RR

    WB result of ACAP1 antibody (82967-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells.

  • Western Blot (WB) analysis of Jurkat cells using ACAP1 Recombinant antibody (82967-1-RR)

    WB analysis of Jurkat using 82967-1-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 82967-1-RR (ACAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ACAP1 Recombinant antibody (82967-1-RR)

    WB analysis of K-562 using 82967-1-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 82967-1-RR (ACAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ACAP1 Recombinant antibody (82967-1-RR)

    IF Staining of Jurkat using 82967-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACAP1 antibody (82967-1-RR, Clone: 230370E1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 82967-1-RR

    Affinity and Kinetic Characterization of 82967-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82967-1-RR against Human ACAP1 were performed. The affinity constant is 0.42 nM.

  • Featured Product
  • KD/KO Validated

ACAP1 Recombinant monoclonal antibody

ACAP1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82967-1-RR
Clone No.230370E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 750
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82967-1-RR targets ACAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34309

Product name: Recombinant human ACAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 163-270 aa of BC018543

Sequence: RAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEG

相同性解析による交差性が予測される生物種
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Calculated molecular weight 740 aa, 82 kDa
Observed molecular weight 82-85 kDa
GenBank accession numberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
RRIDAB_3670713
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15027
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

Protocols

Product Specific Protocols
IF protocol for ACAP1 antibody 82967-1-RRDownload protocol
WB protocol for ACAP1 antibody 82967-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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