Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293T cells, A431 cells, L02 cells, mouse liver tissue | 
| Positive IF/ICC detected in | A549 cells | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85889-1-RR targets ACADVL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse | 
| Host / Isotype | Rabbit / IgG | 
| Class | Recombinant | 
| Type | Antibody | 
| Immunogen | CatNo: Ag6016 Product name: Recombinant human ACADVL protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 312-655 aa of BC012912 Sequence: FDGVRVPSENVLGEVGSGFKVAMHILNNGRFGMAAALAGTMRGIIAKAVDHATNRTQFGEKIHNFGLIQEKLARMVMLQYVTESMAYMVSANMDQGATDFQIEAAISKIFGSEAAWKVTDECIQIMGGMGFMKEPGVERVLRDLRIFRIFEGTNDILRLFVALQGCMDKGKELSGLGSALKNPFGNAGLLLGEAGKQLRRRAGLGSGLSLSGLVHPELSRSGELAVRALEQFATVVEAKLIKHKKGIVNEQFLLQRLADGAIDLYAMVVVLSRASRSLSEGHPTAQHEKMLCDTWCIEAAARIREGMAALQSDPWQQELYRNFKSISKALVERGGVVTSNPLGF相同性解析による交差性が予測される生物種 | 
| Full Name | acyl-Coenzyme A dehydrogenase, very long chain | 
| Calculated molecular weight | 70 kDa | 
| Observed molecular weight | 60 kDa | 
| GenBank accession number | BC012912 | 
| Gene Symbol | ACADVL | 
| Gene ID (NCBI) | 37 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Protein A purification | 
| UNIPROT ID | P49748 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACADVL antibody 85889-1-RR | Download protocol | 
| WB protocol for ACADVL antibody 85889-1-RR | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 


